Functional differences in nucleoside and nucleobase transporters expressed on the rabbit corneal epithelial cell line (SIRC) and isolated rabbit cornea
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منابع مشابه
Molecular evidence and functional expression of P-glycoprotein (MDR1) in human and rabbit cornea and corneal epithelial cell lines.
PURPOSE Efflux pumps such as P-glycoprotein (P-gp; MDR1) are believed to be a major barrier to drug delivery. The purpose of this work was to determine whether cornea and corneal epithelial cells expresses the functionally active P-gp efflux pump. METHOD Cultured rabbit primary corneal epithelial cells (rPCECs) and a corneal cell line (Statens Seruminstitut rabbit cornea [SIRC] cells) were se...
متن کاملPhysical and chemical factors affecting the adherence of Pseudomonas aeruginosa to a rabbit cornea cell line (SIRC) cells.
Adherence of Pseudomonas aeruginosa to a Rabbit Cornea Cell Line (SIRC) cells in vitro was examined. Maximal adherence occurred at pH 5. 0-7. 0 and when the bacterial ~~spension at 1. 4x109 /ml was exposed to 2x104/ml SIRC cells at mid-logarithmic growth phase. Adherence was not affected by temperature. In the passage of time, a temporary rise of adherence occurred within 5 min of incubation an...
متن کاملA rabbit corneal epithelial cell line expresses functional platelet-derived growth factor beta-type receptors.
Platelet-derived growth factor (PDGF) is a family of three isoforms termed PDGF-AA, PDGF-AB, or PDGF-BB that induce proliferation in various mesenchymal cells. A rabbit corneal epithelial cell line (SIRC) was chosen to study the effects of the three isoforms of PDGF. These cells express approximately 43,000 PDGF beta-type receptors and less than 2800 alpha-type receptors on their surface. Thus,...
متن کاملComparison of rabbit corneal endothelial cell precursors in the central and peripheral cornea.
PURPOSE To compare the distribution and self-renewal capacity of rabbit corneal endothelial cell precursors in the central and peripheral regions of the cornea. METHODS The corneal endothelium (CE) and Descemet's membrane of New Zealand White rabbit corneas were divided into a peripheral region (6.0-10.0 mm in diameter) and a central region (6.0 mm in diameter). Then a sphere-forming assay wa...
متن کاملActivation of a CFTR-mediated chloride current in a rabbit corneal epithelial cell line.
PURPOSE To determine whether there is gene expression and functional activity of cystic fibrosis transmembrane conductance regulator protein (CFTR) in an SV40-immortalized rabbit corneal epithelial cell line, tRCE. METHODS Both whole-cell and cell-attached patch-clamp techniques were used to examine the biophysical characteristics of the cAMP-dependent chloride current. The molecular identity...
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ژورنال
عنوان ژورنال: AAPS PharmSci
سال: 2003
ISSN: 1522-1059
DOI: 10.1208/ps050215